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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants were being claimed but thorough nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This tutorial outlines further more feasible optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose customers are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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